Figure 2: iMSCs derived from dermal fibroblasts CRL2097 are multipotent and can differentiate into osteoblasts, adipocytes, and chondrocytes.
From: Efficient Generation of Chemically Induced Mesenchymal Stem Cells from Human Dermal Fibroblasts
(A) To detect early osteogenesis, fibroblasts (CRL2097), iMSCs derived from CRL2097 (iMSCs), and BMMSCs were cultured in osteogenic induction medium for 10 days, and the alkaline phosphatase (ALP) activity assay was performed. (B) To detect late osteogenesis, Alizarin Red S staining (ARS) was performed at day 21. (C) To detect adipogenesis, fibroblasts CRL2097, iMSCs, and BMMSCs were cultured in adipogenic induction medium for 21 days and then stained with Oil Red O. Scale bar: 50 μm. (D) The quantification results of the ALP activity, the Alizarin Red S assay, and the Oil Red O staining (n = 6). ****p < 0.0001. (E) The lacunae structure (Hematoxylin and eosin staining, HE) and proteoglycans of cartilage (Alcian Blue staining) were examined to evaluate the ability of iMSCs to differentiate into chondrocytes at day 21. The lacunae are marked by the yellow arrow. Scale bar, 50 μm.
