Figure 3: iMSCs, like BMMSCs, significantly ameliorate LPS-induced lung inflammation in mice.
From: Efficient Generation of Chemically Induced Mesenchymal Stem Cells from Human Dermal Fibroblasts
(A) The experimental scheme for inducing acute lung injury in mice using intratracheal injections of LPS. (B) iMSCs and BMMSCs significantly ameliorate lung inflammation. Representative lung histology at 48 h after LPS-induced acute lung injury. Scale bar: 100 μm. (C) iMSCs, like BMMSCs, efficiently improve the survival rate of LPS-induced acute lung injury. The results are expressed as a percentage of survival (n = 10–12 per group). *p < 0.05, LPS + PBS V.S. (PBS, LPS + iMSCs, or LPS + BMMSCs). #p < 0.05, LPS + Fibroblasts vs. (PBS, LPS + iMSCs, or LPS + BMMSCs). (D) The injury score of LPS-induced acute lung injury and the quantification of the histology at 48 h after LPS-induced acute lung injury. All sections were quantified after digital slide scanning of the whole slide. n = 10–12 each group. The details of calculation were described in method. The injury score used the following criteria: 0, no injury; 1, 25% injury in the field; 2, 50% injury in the field; 3, 75% injury in the field; and 4, diffuse lung injury. *p < 0.05.
