Figure 2: 3A interacts with ACBD3 in mammalian cell.

(a) HeLa cells were transfected with plasmids encoding GFP (Upper) or GFP-3A (Lower). At 24 h after transfection, cells were fixed and stained with anti-ACBD3 antibody (red). (b) 293 T cells were transfected with plasmids encoding GFP or GFP-3A. At 24 h after transfection, cell lysates were immunoprecipitated with antibody against GFP. Samples were then subjected to Western blot analysis with antibodies against ACBD3, GFP and β-actin. (c) RD cells were mock-infected or infected with EV71 at MOI = 5 of PFU/cell. After 8 h, cells were fixed and stained with antibodies against 3A (Green) and ACBD3 (Red). (d) RD cells were mock-infected or infected with EV71 at MOI = 1 of PFU/cell. After 24 h, cell lysates were immunoprecipitated with antibody against ACBD3. Samples were then subjected to Western blot analysis. (e) RD cells were treated as described in (d). At 4, 8, 12, and 24 h, cells lysates were immunoprecipitated with antibody against 3A. (f) RD cells were mock-infected or infected with EV71 including three different strains as indicated. After 24 h, cell lysates were immunoprecipitated with antibody against ACBD3.