Figure 5: ACBD3 interacts with 3A at C-terminal amino acids.
From: The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A
(a) Schematic diagram of different variants of 3A. (b) 293 T cells were transfected with plasmids encoding GFP or GFP-3A variants along with Flag-ACBD3. At 24 h after transfection, lysates were immunoprecipitated using anti-Flag. Samples were subjected to Western blot analysis. (c) Amino acids sequence alignment of 3A protein of EV71, CVB3, CVA17 and PVS2. (d) 293 T cells were transfected with plasmids encoding GFP or GFP 3A amino acid substitution mutants along with Flag-ACBD3, the immunoprecipitation were done as described above. (e) RD cells were transfected with EV71 RNA transcripts. At 72 h post transfection, cells were harvested and resolved with 12% SDS-PAGE. Western blot analysis for EV71 or β-actin was conducted.
