Figure 7: STK16 depletion or kinase activity inhibition delays mitotic entry and causes prolonged mitosis.
From: STK16 regulates actin dynamics to control Golgi organization and cell cycle
HeLa cells were plated and treated with siRNA or STK16-IN-1 and then subjected to double thymidine synchronization. (a,b) Cells were released from thymidine for 12 or 14 hours before they were harvested for immunofluorescence. (c,d) The percentage of cells in mitosis (mitotic index) was determined by staining DNA with DAPI and an anti-phospho-Histone H3 antibody at 0, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26 and 28 hours after thymidine release for control and STK16 siRNAs transfected cells, or for DMSO and STK16-IN-1 treated cells. For each time point, >1000 cells were counted. Data show mean value, n = 2. Experiments were repeated two times and representative images are shown. (e) STK16 knockdown or inhibition increases cells with chromosome bridges. Representative images of DNA in siNegative, siSTK16, or in DMSO or STK16-IN-1 treated cells. Scale bar, 10 μm.
