Figure 8: STK16 affects mitotic progression.
From: STK16 regulates actin dynamics to control Golgi organization and cell cycle
(a,b) Quantification of cells in mitosis and cytokinesis, cells in each cell cycle stage during mitosis and cytokinesis in HeLa, HeLa-STK16-GFP-FLAG-WT (WT), and HeLa-STK16-GFP-FLAG-MUT (RNAi resistant mutant, RNAi-X MUT) cells transfected with siSTK16. (c,d) Quantification of cells in mitosis and cytokinesis, cells in each cell cycle stage during mitosis and cytokinesis in HeLa and HeLa-STK16-GFP-FLAG-WT cells treated with 5 μM STK16-IN-1 for 72 hours. (e) Actin spikes and membrane blebbing in cytokinesis cells are reduced after STK16 knockdown. HeLa cells stably expressing STK16-GFP-FLAG-WT were transfected with control or siSTK16 for 3 days before they were fixed and stained by phalloidin and anti-GFP. Experiments were repeated three times and representative images are shown. The data were analyzed using a student’s t- test (*p < 0.05, **p < 0.01, ***p < 0.001). Data show mean ± SEM, n = 3. Scale bar, 10 μm.
