Figure 5: ATP catabolism by BMN and BMDM in vitro.
From: Role of purinergic signaling in experimental pneumococcal meningitis
S. pneumoniae was grown in the absence or presence of murine BMDM (a) or BMN (b) (100,000 cells per well) in artificial CSF to which ATP was added at its physiological concentration. After 30 min (grey bars) and 120 min (black bars) of culture, artificial CSF samples were taken and used for ATP determinations using a commercially available bioluminescence kit. When indicated, the ectonucleotidase inhibitor ARL67156 (1 mM) was given to the culture medium prior to the addition of S. pneumoniae and murine immune cells. All experiments were – at least - carried out twice in triplicates. Data are given as means ± SD. **P < 0.001 und *P < 0.01, compared to respective group, using ANOVA and Student-Newman-Keuls post-hoc test.
