Figure 1: Schematic diagram and flowchart of OEPR cloning for large fragment insertion. | Scientific Reports

Figure 1: Schematic diagram and flowchart of OEPR cloning for large fragment insertion.

From: OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments

Figure 1

The cloning method requires only three steps: (A) amplification of insert fragments using primer F1 and R1; (B) exponential amplification using fragments generated in the first PCR and reverse primer R; and (C) digestion by DpnI to eliminate the parent DNA and direct transformation into chemically competent Top10F’ cells.

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