Figure 2: Schematic diagram and flowchart of OEPR cloning for insertion of multiple fragments. | Scientific Reports

Figure 2: Schematic diagram and flowchart of OEPR cloning for insertion of multiple fragments.

From: OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments

Figure 2

The cloning method requires three procedures: (A) amplification of insert fragments a, b and c with primers F1 and R1, F2 and R2 and F3 and R3, respectively; (B) exponential amplification using fragments (a, b, and c) generated in the first PCR and reverse primer R; and (C) digestion by DpnI to eliminate the parent DNA and direct transformation into chemically competent Top10F’ cells.

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