Table 1 Bacterial strains and plasmids.
Strains or plasmids | Relevant characteristic (s) | stx genotype | Source or reference |
|---|---|---|---|
Strains | |||
O157:H7 | Enterohemorragic E. coli O157:H7 reference strain EDL933 | stx1 + stx2+ | ATCC 43895 |
O157:H7-ΔlpfAOI-141 | EDL933 isogenic mutant with lpfAOI-141 gene deleted, KmR | stx1 + stx2+ | Mutant generated in this study |
O157:H7-ΔlpfAOI-154 | EDL933 isogenic mutant with lpfAOI-154 gene deleted, KmR | stx1 + stx2+ | Mutant generated in this study |
O157:H7-ΔlpfAOI-141/ΔlpfAOI-154 | EDL933 isogenic mutant with lpfAOI-141 and lpfAOI-154 genes deleted, KmR | stx1 + stx2+ | Mutant generated in this study |
O157:H7-Δstx2 | EDL933 isogenic mutant with stx2 gene deleted, KmR | stx1 + stx2− | |
O157:H7-ΔfliC | EDL933 isogenic mutant with flagellar gene fliC deleted, KmR | stx1 + stx2+ | |
O6:H10 | Enterohemorragic E. coli O6:H10 strain NV110, naturally lacking lpf genes | stx1 − stx2+ | |
STEC collection | 236 STEC strains isolated from cattle, food and patients | NA | |
K-12 C600 | Non pathogenic E. coli | NA | Laboratory |
Plasmids | |||
pKOBEG | pBAD cloning vector harboring λ phage redγβα operon, CmR | NA | |
pBAD24 | Cloning vector with arabinose inducible promoter, AmpR | NA | |
pBAD33 | Cloning vector with arabinose inducible promoter, CmR | NA | |
pBAD24-lpfAOI-141 | pBAD24 harboring the 537-bp NcoI-PstI fragment containing the entire lpfAOI-141 gene | NA | Plasmid generated in this study |
pBAD33-lpfAOI-154 | pBAD33 harboring the 603-bp HindIII-XbaI fragment containing the entire lpfAOI-154 gene | NA | Plasmid generated in this study |
