Figure 10: Effect of TNF-α and PA on insulin sensitivity and FATP4 expression in mouse heart.
From: TNF-α stimulates endothelial palmitic acid transcytosis and promotes insulin resistance
Mice were assigned to five groups: Ctr, TNF-α, PA, TNF-α+PA, TNF-α+PA+3-MA, and TNF-α+PA+PDTC. (A–C) PET scanning of mice after PA gavage. Representative PET 18F-FDG images (A), post-PET measurement of radioactivity in hearts (B) and in vitro gamma-count heart biodistribution (C) are shown. (D) Western blot analysis of insulin signaling in hearts. *P < 0.05 or **P < 0.01 versus control (Ctr); #P < 0.05 or ##P < 0.01 versus TNF-α; &P < 0.05 or &&P < 0.01 versus PA; †P < 0.05 or ††P < 0.01 versus TNF-α+PA, by one-way ANOVA, (n = 4). (E) Immunohistochemistry for FATP4 expression in hearts.
