Figure 3: DEK is necessary for the activation of γH2AX after ionizing radiation.
From: DEK is required for homologous recombination repair of DNA breaks
(a) MEFs were treated with 10 Gy of gamma ionizing radiation (IR) and stained for γH2AX. Representative cross sections from z-stack confocal images are shown. (b) Quantification of cells with >10 γH2AX foci in (A). At least 50 cells per time point were counted in each biological replicate, and all foci within the z-stack were quantified. DEK−/− MEFs were unable to activate γH2AX above baseline after irradiation. (paired t-test, n = 3, mean ± SEM). (c) Quantification of the average total number of γH2AX foci in each cell from (A). (paired t-test, n = 3, mean ± SEM) The number of foci per cell remained unchanged in DEK−/− MEFs after irradiation. (d) Western blot analysis of γH2AX in MEFs at 6 hs following irradiation with 10 Gy. (e) Western blot analysis of C33A human cancer cells infected with adenovirus 48 hr prior to receiving 10 Gy IR (see also Supplementary Fig. S1).
