Figure 4: Inhibitory effect of IDPP on secretion of infectious HCVcc.

(A) Schematic representation of the viral supernatant transfer experiment. Viral cell culture supernatants were transferred to naïve target cells and analyzed at 3 days post-infection. (B) Black circles, blue squares, and red circles indicate inhibition of viral replication, secretion of infectious HCV, and cell viability, respectively. Results are shown as the mean ± SD of two independent experiments in quadruplicate. Sucrose density gradient ultracentrifugation of cell lysates (C) and cell culture supernatants (D) obtained from transiently transfected HCV producer cells treated with IDPP or untreated (as described in supplement). Data represent relative HCV infectivity to DMSO (untreated control), HCV RNA levels, and buoyant density of each fraction. The mean value of two independent experiments is plotted and statistical significance of changes in HCV particle density calculated (**p < 0.01). (E) Total amounts of intra/extracellular HCV core in the transiently transfected cells were assessed by HCV core ELISA. Experiments conducted in duplicate and normalized to the untreated control. **p < 0.01.