Figure 4: Distribution of methylated cytosine in the 5′ region and exon 1 of the three TaGS2 homoeologs. | Scientific Reports

Figure 4: Distribution of methylated cytosine in the 5′ region and exon 1 of the three TaGS2 homoeologs.

From: Chromatin modification contributes to the expression divergence of three TaGS2 homoeologs in hexaploid wheat

Figure 4

(A) to (C) Leaves of 10-d-old seedlings. (D) to (F) Booting-stage leaves. (G) to (I) Roots of 10-d-old seedlings. (A,D) and (G) TaGS2-A. (B,E) and (H) TaGS2-B. (C,F) and (I) TaGS2-D. The methylation status of CG/CHG/CHH sites in the 5′ regions and exon 1 of TaGS2 homoeologous genes in different tissues and developmental stages. Genomic DNA was treated with sodium bisulfite and used for PCR with primers that amplified fragments in the 5′ regions and exon 1 of the three TaGS2 homoeologous genes. The sequences were determined in 16 clones of each gene. Sequencing data were analyzed using Kismeth software (http://katahdin.mssm.edu/kismeth/revpage.pl). The colored lines above the x-axis show the percentage of methylation at individual cytosine sites. The short bars at the bottom of the graph show the positions of the cytosines.

Back to article page