Figure 5: Accessibility changes of three TaGS2 homoeologs to endonuclease in the roots and leaves.

The nuclei were digested with micrococcal nuclease and DNase I for an increasing time period as indicated. The amount of DNA was detected with quantitative PCR using primers specific for three TaGS2 homoeologs at the promoter and transcribed regions, respectively. Roots and leaves of 10-d-old seedlings and booting-stage leaves were measured. The data are the means ± SD of three independent biological replicates. (A) and (B) Degradation rates of three TaGS2 homoeologs with DNase I treatment. (C) and (D) Degradation rates of three TaGS2 homoeologs with micrococcal nuclease treatment.