Figure 3: Polarization of tumor-associated macrophages (TAMs) and their effects on LLC1 cell proliferation. | Scientific Reports

Figure 3: Polarization of tumor-associated macrophages (TAMs) and their effects on LLC1 cell proliferation.

From: Role of Cyclooxygenase-2 on Intermittent Hypoxia-Induced Lung Tumor Malignancy in a Mouse Model of Sleep Apnea

Figure 3

(a) Assessment of CD206 (M2 phenotype marker, upper) and CD86 (M1 phenotype marker, lower) in TAMs by FACS as well as representative mean fluorescence intensity (MFI) histograms of each marker in N, N+Ce, IH and IH+Ce samples. Up-regulation of M2 marker was observed in TAMs from mice that underwent IH treatment, whereas Ce resulted effective avoiding M2-like polarization. M2/M1 ratio calculated from the quotient between CD206 and CD86 MFIs increased consistently in IH-exposed animals, and was prevented in IH-treated mice that received Ce (center). (c) Proliferation of LLC1 cells co-cultured with TAMs isolated from mice. LLC1 cells increased their proliferation when co-cultured with TAMs obtained from IH-exposed mice, whereas IH+Ce TAMs significantly reduced LLC1 cell proliferation respect to the IH-treated group. Representative dot plot depicting the gating performed to identify CD45+, CD11b+ (TAMs) and CD45−, CD11b− (LLC1) populations during FACS analysis. Data are presented as mean ± SE, MFI = Mean fluorescence intensity (arbitrary units).

Back to article page