Figure 1: Assay of TTR tetrameric stability by IEF. | Scientific Reports

Figure 1: Assay of TTR tetrameric stability by IEF.

From: Cavity filling mutations at the thyroxine-binding site dramatically increase transthyretin stability and prevent its aggregation

Figure 1

(A) Plasma TTR from heterozygote carriers of TTR variants or from controls were isolated by native PAGE electrophoresis and then separated by IEF in semi-dissociating conditions (4 M urea). This is a representative picture selected from the 3 replicas considered for the quantification presented in (B). (B) Relative abundance of tetramer was calculated based on the ratio of intensity of tetramer bands (% Tetramer) over intensity of all TTR bands (Total TTR) in samples from plasma. *P < 0.05 (Student’s t-test). Error bars indicate SD.

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