Figure 4: Aggregation: Samples were incubated at 3.5 μm at pH 4,4 and 37 °C with no agitation. | Scientific Reports

Figure 4: Aggregation: Samples were incubated at 3.5 μm at pH 4,4 and 37 °C with no agitation.

From: Cavity filling mutations at the thyroxine-binding site dramatically increase transthyretin stability and prevent its aggregation

Figure 4

Turbidity at 340 nm was monitored along time to measure aggregation (A). At the end of the experiment, a Th-T assay was performed to confirm the amyloid character of the aggregation (B). Exciting the samples at 450 and collecting the emission from 460 to 600 nm we obtained Th-T spectra. (C) shows TEM of the end point of the aggregated samples. Scale bars values are indicated.

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