Figure 3: Minocycline feeding suppresses insulin/TOR signaling in larval tissues. | Scientific Reports

Figure 3: Minocycline feeding suppresses insulin/TOR signaling in larval tissues.

From: Minocycline treatment suppresses juvenile development and growth by attenuating insulin/TOR signaling in Drosophila animal model

Figure 3

(a) The amounts of phosopho-Akt (p-Akt), Akt, phospho-S6K (p-S6K), and beta actin (b-actin) were measured by western blot using lysates from early 3rd instar larval bodies (72 h AEL) of w1118. Minocycline treatment significantly decreases the amounts of p-Akt, Akt, and p-S6K. (b–d) Quantification of replicated results from the western blot analyses. (e) The mRNA levels of Akt (FBgn0010379) and S6K (FBgn0283472) were measured by RT-PCR. We isolated total RNA from ten early 3rd instar larvae (72 h AEL). Minocycline treatment does not affect the Akt and S6K mRNA levels. (f) Measurement of mRNA levels of Akt (FBgn0010379) and S6K (FBgn0283472) using qRT-PCR. At least three biological replicates from each of the samples were analyzed and the data are presented as mean ± S.D. *p < 0.05 **p < 0.01 when compared to the respective controls (t-test).

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