Figure 8: Comparative Real Time PCR validations of selected genes in mechanically wounded and simulated herbivory wounded chickpea leaves. | Scientific Reports

Figure 8: Comparative Real Time PCR validations of selected genes in mechanically wounded and simulated herbivory wounded chickpea leaves.

From: Simulated herbivory in chickpea causes rapid changes in defense pathways and hormonal transcription networks of JA/ethylene/GA/auxin within minutes of wounding

Figure 8

Expression was studied at 0, 5, 20, 60 and 120 min after mechanical wounding and compared to 0, 5 and 20 min patterns of simulated herbivory wounding (saliva treated) with 0 min being the unwounded control in both set of experiments. The real time expression data was normalized using two validated reference genes EF1alpha and HSP-90. Error bars represent ±SE of three biological replicates. Expression values were analyzed by one-way ANOVA and compared using Duncan’s Multiple Range Test (DMRT). Values on the bar carrying different letters are significantly different (α = 0.05).

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