Figure 2: The effect of OligoG CF-5/20 on bacterial cell membrane permeabilisation. | Scientific Reports

Figure 2: The effect of OligoG CF-5/20 on bacterial cell membrane permeabilisation.

From: The antimicrobial effects of the alginate oligomer OligoG CF-5/20 are independent of direct bacterial cell membrane disruption

Figure 2

Cell permeability assay showing (a) release of carboxyfluorescein (Cbfl) from single lamellar liposomes composed of egg PC:PG (80:20) in the presence of RTA3 (0.5 μM; positive control) or OligoG CF-5/20 at 20–100 mg/ml (b) zoomed-in graph of OligoG CF-5/20 only data. Internalisation of (c) propidium iodide (PI) and (d) nitrocefin (NFN) by PAO1 compared to EDTA (positive) control (1 mM-20 mM). (e) Internalisation of 1-N-phenylnaphthylamine (NPN) dye by P. aeruginosa strains; PAO1, V2 and NH57388A ± 2–20 mg/ml OligoG CF-5/20 and polymyxin B (positive control). (AU = arbitrary units). (Data represents mean ± SD; **p < 0.01 and ****p < 0.0001 compared to control; n = 3).

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