Figure 3: Drg1-21 shows a dysregulated ATPase activity.

Drg1 wildtype as well as mutant variants were purified and the in vitro ATPase activity was measured using the Malachite green phosphate assay. The ATPase activity was determined in the absence (basal activity) and in the presence (stimulated activity) of the HIS₆-tagged C-terminal fragment of the substrate protein Rlp24 (amino acids 147-199). The ATPase activity was measured for proteins purified in the absence (a) or presence (b) of 1 mM ADP. All values are presented as specific ATPase activity (μmol ATP h⁻¹ mg⁻¹ Drg1). Error bars represent standard deviations of means calculated from at least two biological replicates each measured in triplicate. P-values were calculated by an unpaired t-test (n.s.: not significant; p > 0.05).