Figure 6: The Drg1-21 exchanges are located at the N-D1 interface similar to IBMPFD exchanges of p97. | Scientific Reports

Figure 6: The Drg1-21 exchanges are located at the N-D1 interface similar to IBMPFD exchanges of p97.

From: A conserved inter-domain communication mechanism regulates the ATPase activity of the AAA-protein Drg1

Figure 6

(a) Cartoon representation of a modelled Drg1 hexamer in front view (small image) as well as one magnified monomer highlighted in colour (N-terminal domain in yellow, N-D1 linker in black, D1 domain in orange and D2 in red; bound ADP in D1 and D2 in grey and in sphere representation). (b) Magnification of the N-D1 interface with the following residues shown in stick representation: the amino acid substitutions of the Drg1-21 variant (T100I in green and L108P in blue) and the suppressor exchange N77K (magenta). The ribose group of ADP bound in D1 is shown in grey. (c) Domain representation of Drg1 and the related mammalian AAA-ATPase p97. Drg1 residues are coloured according to (b). In p97, red stars mark positions of documented IBMPFD exchanges (annotated according to ref. 30). The “IBMPFD-like” exchange R86A14 is marked in orange. (d) Structural alignment of the N-D1 segments of Drg1 and p97. The exchanges present in Drg1-21 or in p97 IBMPFD variants are coloured as in (c). (e) Magnified section of the overlay of the modelled structure of Drg1 (light grey, transparent) with the structure of p97 (dark grey, solid). Highlighted residues are coloured as in (c).

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