Figure 3: Reduction in size and thickness of Alix ko cortices.
From: Alix is required during development for normal growth of the mouse brain
(a,c) Coronal sections of P0 (a) and adult (c) brains stained with haematoxylin/eosin. Quantifications show that the cortical medio-lateral length (dashed lines) is reduced in P0 and the adult, while the radial thickness (solid lines) is reduced in the adult only (n = 4 animals *p < 0.05; ***p < 0.001). (b,d) Sections of P0 (b) and adult (d) motor cortex labeled with anti-Ctip2, (b) and anti-NeuN (d) antibodies to discriminate cortical layers (LI-LV/VI). Measurements reveal thinning of all layers in adult (d), but only of layer VI in P0 Alix ko brains (b) (n = 7 animals for P0, n = 4 animals for adults *p < 0.05; **p < 0.01; ***p < 0.001).
