Figure 4: Reduced cortical size in Alix ko embryos correlates with a reduced number of intermediate progenitors and neurons.
From: Alix is required during development for normal growth of the mouse brain
(a) DAPI stained coronal sections of E11.5, E12.5, E13.5, E15.5 embryonic brains. Arrowheads and white frames in (a) show examples of regions used for measurements of the medio-lateral length at the ventricule side of the dorsal telencephalon (b) and the cortical thickness (c) (n = 3 embryos per stage *p < 0.05). (d) Single plane confocal image of dorsal telencephalon labeled with DAPI (blue) and anti-Tuj1 (red) (E11.5, E12.5), and Tbr2 (green) (E13.5). (e,f) Reduction in the number of nuclei at E12.5 (e) and in the percentage of Tbr2 + progenitors at E13.5 (f). Counts were performed in a rectangle with a 150 μm base along the ventricle and the height of the cortical wall (n = 3 embryos per stage **p < 0.01). (g) The Tuj1 + neuron layer is thinner in Alix ko E13.5 cortices (n = 3 embryos **p < 0.01).
