Figure 5: Modified cell proliferation does not account for the reduced cortical size of Alix ko brains.
From: Alix is required during development for normal growth of the mouse brain
(a) E12.5 cortices stained with anti-phospho-histone H3 to label M-phase nuclei (pH3, green), and anti-Tuj1 to label neurons (red). Cells were counted in a rectangle with a 100 μm base along the ventricle and the height of the cortical wall (n = 3 embryos). (b) BrdU incorporation is increased in Alix ko cortex. Sections were stained with anti-BrdU antibody. Dashed lines delimit the basal surface of the cortex. Bar chart shows the relative fluorescence intensities (n = 3 embryos *p < 0.05). (c) Flow cytometry of cells freshly dissociated from E12.5 cortices. Left panels show profiles of DNA content of wt and ko cells stained with propidium iodide. The average percentage of cells in each cell cycle phase shows a decrease in G1/ GO (2 N), an increase in S-phase, but no increase in polyploid (4 N and 8 N) cells in Alix ko cortices (n = 3 embryos *p < 0.05; **p < 0.01).
