Figure 9: Functional impairment in growth cones of Alix ko neurons.
From: Alix is required during development for normal growth of the mouse brain
(a) Growth cones of 1DIV neurons stained for F-actin with phalloïdin-Texas red. Quantification shows the reduction in the surface area of growth cones in Alix ko (n = 3 independent experiments **p < 0.01). (b) Relative CTxB endocytosis per growth cone is impaired in Alix ko neurons. Surface bound CTxB was removed and the fluorescence of internalized CTxB quantified. Arrowheads indicate growth cones where endocytosis was quantified (n = 3 independent experiments **p < 0.01).
