Figure 1: Identification of the madC gene of P. blakesleeanus by positional cloning. | Scientific Reports

Figure 1: Identification of the madC gene of P. blakesleeanus by positional cloning.

From: A Ras GTPase associated protein is involved in the phototropic and circadian photobiology responses in fungi

Figure 1

(A) Phenotypes of a wild type and five representative madC mutant strains. The effect of light, illuminated from the left side, on the sporangiophores of the wild type (WT) NRRL1555 and the allelic series of madC strains (1–5) grown in potato dextrose agar medium. (B) Segregation of the madC gene with molecular markers. Progeny (n = 93) from a cross between strains UBC21 × B2 were analyzed using PCR-RFLP markers. Marker names indicate the primers used for the amplification of the regions that are polymorphic between the two parents. Distances are in map units. (C) Position of mutations in the madC gene on a diagram of the gene, in which blue boxes indicate exons. 19 independent madC strains were examined: all bear a mutation along the gene. Numbers 1–5 indicate the alleles identified in the gene. The mutant strains have reduced phototropism and have allele 1: strains A202, A914, B2, B3, B4, S47, S193, S196 and S205; allele 2: strains C148, L1, L72 and S5; allele 3: strains C39, C54 and C93; allele 4: strains A491 and A492; allele 5: strain A905. (D) Nucleotide changes in the madC mutants compared to the wild type sequence. Lower-case letters are intron sequences. All the alleles would produce an impaired protein: alleles 1 and 2 affect the 5′ G of the intron, alleles 3 and 4 introduce premature stop codons (underlined), and allele 5 introduces a new 3′ splicing site.

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