Figure 2: P. blakesleeanus MadC and Ras homologs physically interact in a yeast two-hybrid assay. | Scientific Reports

Figure 2: P. blakesleeanus MadC and Ras homologs physically interact in a yeast two-hybrid assay.

From: A Ras GTPase associated protein is involved in the phototropic and circadian photobiology responses in fungi

Figure 2

S. cerevisiae strain PJ69-4A has the ADE2, HIS3 and β-galactosidase genes under the control of galactose-inducible promoters. Physical interactions between proteins fused to the Gal4 activation domain (in plasmid pGADT7) and DNA binding domain (in plasmid pGBKT7) trigger the expression of these three reporters. (A) Growth of strains co-transformed with two plasmids on –leucine –tryptophan (–leu –trp) to maintain the plasmids, or on media to measure interactions (–histidine + 0.5 mM 3-amino triazole, or –adenine). (B) The average β-galactosidase activities of the strains, with standard errors from two biological replicates. (C) Arrangement of the cDNA clones in the plasmids with the Gal4 activation and DNA binding domains. EV; empty vector.

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