Figure 3: The P. blakesleeanus madC gene complements the heat shock sensitivity of the S. cerevisiae ira1 mutant.

The wild type strain of S. cerevisiae and an ira1Δ strain were transformed with an empty vector or with a vector expressing the madC gene. Ten-fold serial dilutions of the strains were plated on YPD medium. One plate was heat shocked at 50 °C for 120 min. The plates were then cultured 2 days at 30 °C.