Figure 1: Computational-Cannula Microscopy (CCM) in the epi configuration.
From: Deep-brain imaging via epi-fluorescence Computational Cannula Microscopy
(a) Fluorescence from a point source enters the distal end of cannula and is guided via total internal reflections. A pattern is formed at the proximal end of the cannula, which is unique to the location of the point source, i.e., the system exhibits space-variant point-spread functions (SV-PSFs). Three (experimentally measured) SV-PSFs are shown (top) along with the corresponding point source locations (bottom). (b) Schematic of epi-fluorescence CCM. Objective and tube lens are set to image the proximal end of cannula for transferring the image to the sensor. A conventional widefield microscope is placed underneath the sample (only for glass slides) to capture reference images. This is omitted to simplify the diagram. (c) CCM images of fluorescent beads (first row) and cultured DIV 16 hippocampal neurons (second and third row) on glass slides. Left: the reconstructed images, Right: reference images. White dashed line represents the field of view (diameter = 200 μm). (d) Closely spaced micro-beads for resolution test. Region inside the red square is magnified in the second row. The corresponding line-scans through the red dashed lines are shown in the bottom row.
