Figure 6: The effects of PocR or PrpR overexpression on SPI-1 and SPI-4 in the ΔptsN mutant strain in the presence of 1,2-PDL or propionate.
From: Enzyme IIANtr Regulates Salmonella Invasion Via 1,2-Propanediol And Propionate Catabolism
(a) The relative mRNA expression of genes involved in SPI-1 and SPI-4 was determined by qRT-PCR in the wild-type containing pUHE21-2lacIq and in the ΔptsN mutant strains harboring either pUHE21-2lacIq or pPocR. pocR, which encodes the transcriptional activator for the pdu operon, was induced by the addition of 1 mM IPTG from pPocR. Strains were grown in LB medium containing 1,2-PDL (12.5 mM) as an additional carbon source. (b) A similar qRT-PCR was performed, but pPrpR producing PrpR, the transcriptional activator of the prp operon, was used instead, and propionate (10 mM) was added accordingly. Every mRNA level was normalized using that of gyrB and its relative expression to ptsN was averaged from three assays at least.
