Figure 5: Validation of ionChannelΩ screening system with different ligand-gated ion channels. | Scientific Reports

Figure 5: Validation of ionChannelΩ screening system with different ligand-gated ion channels.

From: A new electro-optical approach for conductance measurement: an assay for the study of drugs acting on ligand-gated ion channels

Figure 5

CRCs are obtained as described in Fig. 4 and represented as change in conductance. The semilogarithmic plots for 4 different drug/receptor interactions are shown: capsaicin/h-TRPV1; streptonigrin/h-TRPM2; GABA/h-GABAA; and Bz-ATP/h-P2 × 7 R. Data obtained as in Fig. 4 are converted, represented by a semilogarithmic plot of drug concentrations and fitted with a sigmoid function. R-Sq, as a measure of the goodness-of-fit, and EC50 are reported in the panels. These experiments are representative of at least 7 independent experiments for each condition.

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