Figure 5: miR-24-3p suppressed cancer progression via p130Cas regulation.

(A and B) MCF7_Control and MCF7_EGFP-Cas cells were transfected with either miR-24-3p mimic or control miRNAs and cell migration/invasion was analyzed. After the cells reached confluency, wound closure was determined by measuring cell migration distance (A). Cells were cultured in transwell with or without matrigel, and migrated cells were stained and analyzed by counting cells from three different fields (B). (C) 1 × 10³ of miRNA-transfected cells were cultured for 3 weeks. The colonies were stained with crystal violet and counted in 3 randomly selected visual fields. The images were representative of three independent experiments and graphs indicate the mean ± SEM of three independent experiments. *p < 0.05.