Figure 4: Comparison of the synergistic analogue-digital assay and current state-of-art digital methods.
From: Calibration-free assays on standard real-time PCR devices
(A) Experimental results for 16-compartment multivolume digital (blue points) and synergistic analogue-digital (red points) assays. The value of concentration calculate from the outcome of the assay as a function of a real value of initial concentration is given. Data point shows the averaged results from 12 runs of the assay. The error bars show the standard deviation. The dynamic range of the assays is marked with the gray region. (B) The precision of multivolume digital (blue) and synergistic analogue-digital (red) assays given as the relative standard deviation of the estimates within and outside the dynamic range. The dotted lines show the required precision of the assays. (C) The comparison of the performance of large digital and synergistic assays. To provide 10% precision of the assessment within 6 log dynamic range, multivolume digital assays (blue) require 1120 compartments, while using synergistic scheme (red) requires only 332 compartments. If we use the same number of compartments, the precision of the assessment is improved.
