Figure 3: Ocular fundus images and fluorescein angiography in vivo analysis.

(A) Fundus images were taken weekly, and images from the control group, the untreated DM/hypoxic groups, and the 50 mg/kg shikonin treatment group at days 0 and 28 are represented. The area surrounded by the dotted line represents geographic lesions in the retinas of the untreated DM/hypoxic group at day 28. (B) Fluorescein angiography was performed approximately 30 seconds after intravenous injection of a 10% sodium fluorescein solution. The area in the dotted line shows a hyperfluorescent lesion in retina and red arrows indicate several hypofluorescent areas in the retina of untreated diabetic/hypoxic mice at day 28. Black lines from (A) and (B) indicate the region selected for vertical scan OCT in Fig. 4.