Figure 1: Fucoidan prevents tumorigenesis in LLC1-bearing C57BL/6 mice in vivo.

Mice were pretreated with fucoidan (24 mg/kg) for 14 days before LLC1 cells (2 × 105) were hypodermically inoculated into the dorsum of male C57BL6 mice. On the 15th day, the mice were divided into three groups and processed in a “previous and continuous” fashion (A). In brief, the following groups were established: oral feeding with ddH2O (control), oral feeding with cessation of fucoidan feeding on the day of LLC1 cell inoculation, (EXP 1) and maintained fucoidan feeding for 26 days (EXP 2) at 7 oral feedings/week/mouse. (B) The tumor volumes and (C) tumor samples from the mice were collected at the end of the treatment. (D) The mouse weights were measured as indicated. Three of five individual experiments are presented (n = 5). Each bar represents the mean ± standard deviation (SD), and error bars indicate the SD. Statistically significant differences are shown (*P < 0.05) compared with the control group.