Figure 4: Molecular analysis of MUPs from Mus musculus domesticus and Mus musculus musculus.

Example urinary MUPs from both sexes of (a) domesticus and (b) musculus resolved by native gel electrophoresis, with average intact mass profiles (derived by ESI-MS) presented to the right of gel images. Each electrophoretically resolvable band (labelled A through E) was assigned to one or more MUPs by in-gel digestion, followed by peptide mass fingerprinting by MALDI-TOF mass spectrometry (see Supplementary Figs S2 and S3). The intact mass of each of these MUPs was also confirmed as present in the intact mass profiles.