Figure 4: Acute exposure to LYC altered cellular Ca²⁺ handling in a dose-dependent manner in single cardiomyocytes from healthy mice.

(A) Typical traces of the acute effect of LYC (14 nM) on sarcomere shortening (top panel) and Ca²⁺ transient (bottom panel) in presence of Iso (10 nM) B-F: Averaged acute effects of LYC at 0.14, 1.4 and 14 nM under basal and stress conditions (Iso 10 nM) on sarcomere shortening (B), Ca²⁺ transient amplitude (C), diastolic Ca²⁺ levels (D), and decay of Ca²⁺ transients. (E) Tau (time constant). Ca²⁺ is expressed as the ratio of fluorescence at 405 and 480 nm. (F) Typical traces of Ca²⁺ transient decay under control and Iso conditions. ^‡p < 0.05, vs. Ctrl under the same conditions; ^#p < 0.05, basal vs. stress condition; 10 cells per group.