Figure 5: Acute exposure to LYC induced abnormal diastolic Ca²⁺ waves in a dose-dependent manner in cardiomyocytes from healthy mice.

Representative traces of spontaneous Ca²⁺ waves during resting periods following trains of stimulation in single cardiomyocytes, under basal (A) and stress conditions (Iso 10 nM) (B). (C) Effect of increasing doses of LYC (0.14, 1.4 and 14 nM) on the occurrence of Ca²⁺ waves. Results are expressed as the percentage of cells exhibiting at least one Ca²⁺ wave; *p < 0.05, LYC vs. other groups under the same conditions; ^‡p < 0.05, vs. Ctrl under the same conditions; ^#p < 0.05, basal vs. stress condition; 20–30 cells per group.