Figure 1: H19 expression is elevated under hypoxia and Hif-1α is a critical factor responsible for the induction of H19 RNA in U87 and U251 cells. | Scientific Reports

Figure 1: H19 expression is elevated under hypoxia and Hif-1α is a critical factor responsible for the induction of H19 RNA in U87 and U251 cells.

From: Hypoxia induces H19 expression through direct and indirect Hif-1α activity, promoting oncogenic effects in glioblastoma

Figure 1

The levels of H19 RNA normalized to β-actin were quantified by qPCR. Protein levels were detected by western blot and normalized to β-actin levels. (A) U87 and U251 cells were exposed to hypoxia (2% O2) for the indicated times. H19 levels at each time point (bar graphs) were normalized to normoxic controls. (B) U87 and U251 cells were transfected with Hif-1α expression plasmid or empty vector before culture under hypoxia for 24 h. (C) U87 and U251 cells were transfected with a control siRNA, or Hif-1α siRNA and cultured under hypoxia for 24 h. All experiments were repeated three times with similar results. (*p < 0.05, **p < 0.01). (D) Immunofluorescent double staining was performed on xenograft tumors after U87 cells stably expressing Hif-1α or control vector be subcutaneously injected. The positive expression of Hif-1α and H19 were overlapped in the same section of U87 xenograft tumors.

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