Figure 6: H19 plays an important role in hypoxia-driven migration and invasion in U87 and U251 cells. | Scientific Reports

Figure 6: H19 plays an important role in hypoxia-driven migration and invasion in U87 and U251 cells.

From: Hypoxia induces H19 expression through direct and indirect Hif-1α activity, promoting oncogenic effects in glioblastoma

Figure 6

H19 regulates EMT-related protein expression, affecting migration and invasion in U87 and U251 cells. U87 and U251 cells were transfected with control siRNA or H19 siRNA. The samples are derive from the same experiment and that blots were processed in parallel. (A) Increased H19 expression correlates with glioma grade and confers a poor prognosis in high grade glioma (HGG) and GBM patients. Levels of H19 were analyzed in different glioma tissues of TCGA data. Kaplan–Meier survival curves for H19 expression in HGG and GBM patients of TCGA data. (B) The scratch-wound gap was photographed before and after 24 h incubation under normoxia and hypoxia. After performing the wound healing test. The results were analyzed by measuring the range of migrating cells from 3 different fields for each wound. (C) Transwell migration assays were used to evaluate glioblastoma cell invasion. Migrated cells with normoxic or hypoxic treatment for 24 h were stained with crystal violet and counted for statistical analysis. (D) EMT-related proteins in the three cell groups were examined by western blot and normalized to β-actin. (*p < 0.05, **p < 0.01). The samples are derived from the same experiment and that blots were processed in parallel.

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