Figure 7: H19 affects β-catenin expression by regulating miR-181d activity. | Scientific Reports

Figure 7: H19 affects β-catenin expression by regulating miR-181d activity.

From: Hypoxia induces H19 expression through direct and indirect Hif-1α activity, promoting oncogenic effects in glioblastoma

Figure 7

(A) Bioinformatics predicted a miR-181d binding site in human H19. (B) Ectopic miR-181d expression by miRNA mimics did not affect the H19 levels in U87 cells. (C) U87 cells were co-transfected with miR-181d mimics or miR-16-1 mimics and luciferase reporters harboring the miR-181d binding site sequence or a mutated one from H19. Luciferase activities were normalized to the activities in cells expressing scrambled miRNA mimics. (D) U87 and U251 cells were co-transfected with miR-181d mimics and H19 expression plasmids, β-catenin protein levels were examined by western blot. (*p < 0.05, **p < 0.01). The samples are derived from the same experiment and that blots were processed in parallel.

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