Figure 2: Although knockdown of CHC does not affect VEGF-to-ERK1/2 signalling, dynasore causes a strong inhibitory effect.
From: Dynasore impairs VEGFR2 signalling in an endocytosis-independent manner
(a) HUVECs transfected with siRNAs against clathrin heavy chain were stimulated with VEGF for various time points, lysed and subjected to immunoblotting analysis using antibodies against the phosphorylated or total ERK1/2. Quantification of the effect of clathrin knockdown on the VEGF-induced phosphorylation of ERK1/2 is shown on the right of the immunoblots. The effect is considered non-significant (n = 3, mean ± S.E.M., ANOVA followed by Dunnett’s analysis). (b) Serum starved (2 h) HUVECs were treated with vehicle or dynasore (100 μM), stimulated with VEGF for various time points, lysed and subjected to immunoblotting analysis using antibodies against the phosphorylated or total ERK1/2. Quantification of the effect of dynasore treatment on the VEGF-induced phosphorylation of ERK1/2 is shown on the right of the immunoblots (n = 3, mean ± S.E.M., P < 0.001, ANOVA followed by Dunnett’s analysis).
