Figure 4: Dynasore inhibits signalling and endocytosis with different efficacies.
From: Dynasore impairs VEGFR2 signalling in an endocytosis-independent manner
(a) Immunoblotting analysis of the dose-dependent effect of dynasore on the VEGF-induced phosphorylation of ERK1/2 in HUVECs. Quantification of the phospho-ERK1/2 zones is shown on the right of the immunoblots (n = 3, mean ± S.D., **P < 0.05, ***P < 0.001, t-test). (b) Analysis of the dose-dependent effect of dynasore on CME. HUVECs were pre-treated (30 min) with different concentrations of dynasore and incubated with FITC-transferrin for 15 min, acid-washed, fixed and processed for immunofluorescence microscopy analysis (15 microscopy fields from 3 independent experiments were analysed, mean ± S.D., *P < 0.05, **P < 0.005, ***P < 0.001, t-test). (c) Dose response comparison of the inhibitory effect of dynasore on transferrin internalisation versus inhibition of VEGF-to-ERK1/2 phosphorylation. Graph bars express the % of internalised transferrin, or ERK1/2 phosphorylation, of dynasore treated cells relatively to vehicle treated cells.
