Figure 6: MS-275 restored the in vivo mRNA levels of IL10 in macrophages but did not affect the CD68⁺ or CD68⁺/IL-10⁺ cell number in lungs of cigarette smoke-exposed mice.

MS-275 restored IL10 mRNA levels of cigarette smoke-exposed mice to mRNA levels of air-exposed mice (A). Data are presented as means ± SD; n = 4–5 animals per group. *p < 0.05 compared to vehicle (smoke and inhibitor solvent-treated) mice. Numbers of CD68⁺ and CD68⁺/IL-10⁺ macrophages in the lungs were determined by immunohistochemistry (B). Images were taken with identical instrumental conditions and representative images of 4–5 independent mice per group are shown (original magnification 200x). Immunohistochemical stainings were quantified manually and presented as number/mm² lung tissue (C). Scatter plots showing an increase in CD68⁺ macrophages upon cigarette smoke exposure. CD68⁺/IL-10⁺ macrophages remained unaffected. MS-275 did not affect the influx of CD68⁺ or CD68⁺/IL-10⁺ macrophages. Data are presented as mean ± SD; n = 4–5 animals per group. *p < 0.05 compared to air-exposed animals.