Figure 1: Experimental workflow.

P40/50 and P50/70 cells were isolated from muscles of 4-day-old piglets. Gene expression (via qRT-PCR) was analysed in freshly isolated cells. Proliferation rate was measured when cells were passaged at day 4, 8 and 14. Cell morphology and size during proliferation, growth behaviour, protein expression (via flow cytometry and immunofluorescence), bioenergetic profile and differentiation potential were monitored at given time points.