Figure 2: Gene expression analysis of freshly isolated P40/50 and P50/70 cells. | Scientific Reports

Figure 2: Gene expression analysis of freshly isolated P40/50 and P50/70 cells.

From: Molecular and functional heterogeneity of early postnatal porcine satellite cell populations is associated with bioenergetic profile

Figure 2

Gene expression analysis of the myogenic marker genes Pax7, Myf5, MyoD, Desmin, MyoG, and embryonic Myosin (eMyH) of freshly isolated cells from LD muscle (n = 6). Quantitative real-time PCR shows the higher expression of transcription factors Myf5 and MyoG in P40/50 cells. In contrast, Desmin and eMyH are significantly upregulated in P50/70 cells. ΔΔCT values of each sample are presented as Box-Whisker plots with the maximum 1.5 of the interquartile range (Q1–Q3), and the resulting outliers are included as circles. For statistical analysis, Students t-test (Pax7, MyoD, Desmin, MyoG, eMyH) or Mann-Whitney Rank Sum Test (Myf5) was performed, **p ≤ 0.01, ***p ≤ 0.001.

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