Figure 5: SC in P40/50 population preferentially differentiates compared with P50/70 cells. | Scientific Reports

Figure 5: SC in P40/50 population preferentially differentiates compared with P50/70 cells.

From: Molecular and functional heterogeneity of early postnatal porcine satellite cell populations is associated with bioenergetic profile

Figure 5

Light microscopy of differentiating SC isolated from SM muscle (left). Cells were seeded at 8 or 14 days after isolation in growth medium. Before reaching confluency, cells from both subpopulations were transferred to differentiation medium to allow differentiation. P40/50 cells show a more elongated and dense morphology than P50/70 cells (see especially in the lower panel). Scale bar represents 50 μm. Fusion rate (right) of cells isolated from SM and LD muscle was determined during early differentiation (7 days after the start of the differentiation assay) as the percentage of total nuclei being localized in fused multinucleated cells (day 8: n = 8, day 14: n = 9). P40/50 cells show significantly higher cell fusion compared with P50/70 cells. For statistical analysis, Students two-tailed t-test was performed, *p ≤ 0.05, ***p ≤ 0.001.

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