Figure 4: Effect of TC-PTP deficiency on TPA-induced epidermal proliferation.
(A) Representative photomicrographs of primary keratinocytes from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice after days 1–3 of culture. Equivalent number of primary keratinocytes from both genotypes were seeded and cultured for 3 days. (B) Proliferation of primary keratinocytes was measured using the WST-assay. *p < 0.05 by Mann-Whitney U test. (C) Primary keratinocytes were treated with TPA (40 nM) for 1 h. Proliferation of primary keratinocytes was measured using WST-assay. (D–I) Groups of 7 weeks old mice (n = 3/group) were treated topically with single application of TPA (6.8 nmol) and sacrificed 24 or 48 h after treatment. (D) Representative hematoxylin and eosin staining of epidermis from both genotypes of mice. Scale bar: 100 μm. (E) Quantification of epidermal thickness from both genotypes treated with TPA. *p < 0.05 by Mann-Whitney U test. (F) Representative BrdU staining of epidermis from both genotypes of mice. Scale bar: 100 μm. (G) Quantification of BrdU-labled keratinocytes from epidermis of both genotypes. *p < 0.05 by Mann-Whitney U test. (H) Representative PCNA staining of epidermis from both genotypes of mice. Scale bar: 100 μm. (I) Quantification of PCNA-positive keratinocytes from epidermis of both genotypes. *p < 0.05 by Mann-Whitney U test.
